LOCUS       Exported                3843 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0065_sensor_pluxRRR12y-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="R2oDNA spacer"
                     /note="R2oDNA 70bp spacer 13"
     terminator      203..230
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(238..308)
                     /note="taR12y"
     promoter        complement(309..363)
                     /note="lux rR (R0062)"
     misc_feature    364..433
                     /note="spacer (B0040)"
     promoter        434..488
                     /note="lux rR (R0062)"
     3'UTR           489..540
                     /note="crR12y"
     RBS             523..534
                     /note="RBS (B0034)"
     CDS             541..1266
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1269..1385
                     /note="T1"
     terminator      1278..1364
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1392..1411
                     /note="AB179"
     primer_bind     1577..1596
                     /note="AB5"
     rep_origin      1730..2557
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1978..1980
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1981..2525)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2629..2648
                     /note="AB180"
     oriT            2659..2767
                     /note="incP origin of transfer"
     misc_feature    2739
                     /note="C->T no real mutation, just for testing"
     CDS             complement(2923..3738)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg
      181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct
      241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg
      301 atttagcttt tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca
      361 ggtaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt
      421 cactgaccta acaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt
      481 cgaataaaga attctaccat tcacctcttg gatttagcta ttaaagagga gaaaggtacc
      541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
      661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
      781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
      961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct
     1261 taataaatta aaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1321 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacag
     1381 ctgggcgcgc caaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1441 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
     1501 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
     1561 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
     1621 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
     1681 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac
     1741 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     1801 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     1861 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     1921 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     1981 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
     2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc
     2101 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     2161 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     2221 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2281 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     2341 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     2401 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     2461 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     2521 ctcaagaaga tcatcttatt aatcagataa aatatttcta gcatgagcgg atacatattt
     2581 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2641 cctgctaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     2701 gtgcgaataa gggacagtga agaaggaaca cccgctcgtg ggtgggccta cttcacctat
     2761 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     2821 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     2881 gcagggttat gcagcggaaa aggacccttg tccaattaat tattagaaaa attcatccag
     2941 catcagatga aattgcagtt tgttcatatc cggattatca atgccatatt tctgaaacag
     3001 acgtttttgc aggctcgggc taaattcgcc caggcagttc cacagaatgg ccagatcctg
     3061 ataacgatcc gcaatgccca cacggcccac atcaatgcag ccaatcagtt tgccttcatc
     3121 gaaaatcagg ttatccaggc taaaatcgcc gtgggtcacc acgctatccg ggctaaacgg
     3181 cagcagttta tgcatttctt tccacacctg ttccaccggc cagccgttac gttcatcatc
     3241 aaaatcgctc gcatccacca ggccgttgtt catacggctc tgcgcctggg ccagacgaaa
     3301 cacacgatcg ctgttaaacg ggcagttgca caccggaatg ctatgcagac gacgcagaaa
     3361 cacggccagc gcatccacaa tgttttcgcc gctatccgga tattcttcca gcacctgaaa
     3421 cgcggttttg cccggaatcg cggtggtcag cagccacgca tcatccgggg tgcgaataaa
     3481 atgtttaatg gtcggcagcg gcataaattc ggtcagccag ttcagacgca ccatttcatc
     3541 ggtcacatcg ttcgccacgc tgcctttgcc atgtttcaga aacagttccg gcgcatccgg
     3601 tttgccatac agacgataaa tggtcgcgcc gctctgaccc acgttatcac gcgcccattt
     3661 atagccatac agatccgcat ccatgttgct gttcagacgc ggacggctac agctcgtttc
     3721 acgctgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     3781 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaaa
     3841 ttt
//
